This Rat High sensitivity C-Reactive Protein (hs-CRP) ELISA Kit employs a two-site sandwich ELISA to quantitate hs-CRP in samples. An antibody specific for hs-CRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyhs-CRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for hs-CRP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of hs-CRP bound in the initial step. The color development is stopped and the intensity of the color is measured.
* VAT and and shipping costs not included. Errors and price changes excepted
