Rat Insulin (INS) ELISA Kit employs a two-site sandwich ELISA to quantitate Insulin in samples. An antibody specific for Insulin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Insulin present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Rat Insulin detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Insulin bound in the initial step. The color development is stopped and the intensity of the color is measured.
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