HE4, EDDM4, WFDC2, MGC57529, WAP5, dJ461P17.6, WAP domain containing protein HE4-V4, epididymal protein 4, epididymal secretory protein E4, epididymis-specific, whey-acidic protein type, four-disulfide core, m
This Human Epididymal protein 4 (HE4) ELISA Kit employs a two-site sandwich ELISA to quantitate HE4.
This Human Epididymal protein 4 (HE4) ELISA Kit employs a two-site sandwich ELISA to quantitate HE4 in samples. An antibody specific for HE4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHE4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HE4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HE4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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