This Human Von Willebrand Factor (VWF) ELISA Kit employs a two-site sandwich ELISA to quantitate VWF in samples. An antibody specific for VWF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVWF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VWF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VWF bound in the initial step. The color development is stopped and the intensity of the color is measured.
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