EliKine(TM) Human IFN-gamma ELISA Kit

Product Overview

• Article Name: EliKine(TM) Human IFN-gamma ELISA Kit
• Biozol Catalog Number: ASC-KTE6011
• Supplier Catalog Number: KTE6011
• Alternative Catalog Number: ASC-KTE6011-48, ASC-KTE6011-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: IFG, IFI, IFN gamma, IFN Immune, IFNG, Interferon gamma, Interferon Gamma Precursor, Macrophage Activating Factor, MAF, T Cell Interferon, Type II Interferon

Product Description

EliKine(TM) Human IFN-gamma ELISA Kit employs a two-site sandwich ELISA to quantitate Human IFN-gamma.

Product Properties

• Range: 7.8 pg/mL-500 pg/mL
• Sensitivity: 4 pg/mL
• Tag: IFNG
• NCBI: 3458
• UniProt: P01579
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: EliKine(TM) Human IFN-gamma ELISA Kit employs a two-site sandwich ELISA to quantitate IFN-gamma in samples. An antibody specific for IFN-gamma has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-gamma present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-gamma is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-gamma bound in the initial step. The color development is stopped and the intensity of the color is measured.