EliKine(TM) Human IL-5 ELISA Kit

Catalog Number: ASC-KTE6016
Article Name: EliKine(TM) Human IL-5 ELISA Kit
Biozol Catalog Number: ASC-KTE6016
Supplier Catalog Number: KTE6016
Alternative Catalog Number: ASC-KTE6016-48, ASC-KTE6016-96
Manufacturer: Abbkine Scientific
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: IL 5, IL5, IL-5, IL-5T-cell replacing factor, interleukin-5, TRF, TRFB cell differentiation factor I
EliKine(TM) Human IL-5 ELISA Kit employs a two-site sandwich ELISA to quantitate Human IL-5.
Range: 7.8 pg/mL-500 pg/mL
Sensitivity: 4 pg/mL
Tag: IL5
NCBI: 3567
UniProt: P05113
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: EliKine(TM) Human IL-5 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-5 in samples. An antibody specific for IL-5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-5 is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound in the initial step. The color development is stopped and the intensity of the color is measured.