EliKine(TM) Human IL-10 ELISA Kit

Catalog Number: ASC-KTE6019
Article Name: EliKine(TM) Human IL-10 ELISA Kit
Biozol Catalog Number: ASC-KTE6019
Supplier Catalog Number: KTE6019
Alternative Catalog Number: ASC-KTE6019-48, ASC-KTE6019-96
Manufacturer: Abbkine Scientific
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: IL 10, IL10, CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
EliKine(TM) Human IL-10 ELISA Kit employs a two-site sandwich ELISA to quantitate Human IL-10.
Range: 2.35 pg/ml-150 pg/ml
Sensitivity: 1 pg/mL
Tag: IL10
NCBI: 3586
UniProt: P22301
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: EliKine(TM) Human IL-10 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-10 in samples. An antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-10 is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.