EliKine(TM) Human IL-12 p70 ELISA Kit

Catalog Number: ASC-KTE6020
Article Name: EliKine(TM) Human IL-12 p70 ELISA Kit
Biozol Catalog Number: ASC-KTE6020
Supplier Catalog Number: KTE6020
Alternative Catalog Number: ASC-KTE6020-48, ASC-KTE6020-96
Manufacturer: Abbkine Scientific
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: IL12, IL 12, IL-12, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain
EliKine(TM) Human IL-12 p70 ELISA Kit employs a two-site sandwich ELISA to quantitate Human IL-12 p70.
Range: 7.8 pg/mL-500 pg/mL
Sensitivity: 4 pg/mL
Tag: IL12
NCBI: 3592
UniProt: P29459
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: EliKine(TM) Human IL-12 p70 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-12 in samples. An antibody specific for IL-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-12 is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.