This Human Protein TMEM155 (TMEM155) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM155 in samples. An antibody specific for TMEM155 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM155 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM155 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM155 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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