Human Sex-determining region Y protein (SRY) ELISA Kit

Catalog Number: ASC-KTE60365
Article Name: Human Sex-determining region Y protein (SRY) ELISA Kit
Biozol Catalog Number: ASC-KTE60365
Supplier Catalog Number: KTE60365
Alternative Catalog Number: ASC-KTE60365-48, ASC-KTE60365-96
Manufacturer: Abbkine Scientific
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: SRY, TDF, TDY, essential protein for sex determination in human males, sex determining region protein, sex-determining region on Y, testis-determining factor
This Human Sex-determining region Y protein (SRY) ELISA Kit employs a two-site sandwich ELISA to quantitate SRY.
Range: Please inquire
Sensitivity: Please inquire
Tag: SRY
NCBI: 6736
UniProt: Q05066
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: This Human Sex-determining region Y protein (SRY) ELISA Kit employs a two-site sandwich ELISA to quantitate SRY in samples. An antibody specific for SRY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySRY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRY bound in the initial step. The color development is stopped and the intensity of the color is measured.