Human Mothers against decapentaplegic homolog 5 (SMAD5) ELISA Kit

Product Overview

• Article Name: Human Mothers against decapentaplegic homolog 5 (SMAD5) ELISA Kit
• Biozol Catalog Number: ASC-KTE60572
• Supplier Catalog Number: KTE60572
• Alternative Catalog Number: ASC-KTE60572-48, ASC-KTE60572-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: SMAD5, DKFZp781C1895, DKFZp781O1323, Dwfc, JV5-1, MADH5, MAD homolog 5, MAD, mothers against decapentaplegic homolog 5, SMA- and MAD-related protein 5, SMAD, mothers against DPP homolog 5, mothers against deca

Product Description

This Human Mothers against decapentaplegic homolog 5 (SMAD5) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD5.

Product Properties

• Range: Please inquire
• Sensitivity: Please inquire
• Tag: SMAD5
• NCBI: 4090
• UniProt: Q99717
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: This Human Mothers against decapentaplegic homolog 5 (SMAD5) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD5 in samples. An antibody specific for SMAD5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD5 bound in the initial step. The color development is stopped and the intensity of the color is measured.