Human NAD-dependent deacetylase sirtuin-3, mitochondrial (SIRT3) ELISA Kit

Product Overview

• Article Name: Human NAD-dependent deacetylase sirtuin-3, mitochondrial (SIRT3) ELISA Kit
• Biozol Catalog Number: ASC-KTE60652
• Supplier Catalog Number: KTE60652
• Alternative Catalog Number: ASC-KTE60652-48, ASC-KTE60652-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: SIRT3, SIR2L3, mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, silent mating type information regulation 2, S.cerevisiae, homolog 3, sir2-like 3, sirtuin 3, sirtuin type 3

Product Description

This Human NAD-dependent deacetylase sirtuin-3, mitochondrial (SIRT3) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRT3.

Product Properties

• Range: Please inquire
• Sensitivity: Please inquire
• Tag: SIRT3
• NCBI: 23410
• UniProt: Q9NTG7
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: This Human NAD-dependent deacetylase sirtuin-3, mitochondrial (SIRT3) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRT3 in samples. An antibody specific for SIRT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.