This Human SH3 domain-binding glutamic acid-rich-like protein (SH3BGRL) ELISA Kit employs a two-site sandwich ELISA to quantitate SH3BGRL in samples. An antibody specific for SH3BGRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySH3BGRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SH3BGRL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SH3BGRL bound in the initial step. The color development is stopped and the intensity of the color is measured.
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