Human Neuroendocrine protein 7B2 (SCG5) ELISA Kit employs a two-site sandwich ELISA to quantitate SCG5 in samples. An antibody specific for SCG5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SCG5 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human SCG5 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SCG5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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