This Human 5-hydroxyeicosatetraenoic acid (5-HETE) ELISA Kit employs a two-site sandwich ELISA to quantitate 5-HETE in samples. An antibody specific for 5-HETE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any5-HETE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5-HETE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5-HETE bound in the initial step. The color development is stopped and the intensity of the color is measured.
* VAT and and shipping costs not included. Errors and price changes excepted
