Human ADAM metallopeptidase with thrombospondin type 1 motif4 (ADAMTS4) ELISA Kit

Catalog Number: ASC-KTE60920
Article Name: Human ADAM metallopeptidase with thrombospondin type 1 motif4 (ADAMTS4) ELISA Kit
Biozol Catalog Number: ASC-KTE60920
Supplier Catalog Number: KTE60920
Alternative Catalog Number: ASC-KTE60920-48, ASC-KTE60920-96
Manufacturer: Abbkine Scientific
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: ADAMTS4, ADAMTS-2, ADAMTS-4, ADMP-1, KIAA0688, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4, aggrecanase-1
This Human ADAM metallopeptidase with thrombospondin type 1 motif4 (ADAMTS4) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTS4.
Range: Please inquire
Sensitivity: Please inquire
Tag: ADAMTS4
NCBI: 9507
UniProt: O75173
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: This Human ADAM metallopeptidase with thrombospondin type 1 motif4 (ADAMTS4) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTS4 in samples. An antibody specific for ADAMTS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTS4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS4 bound in the initial step. The color development is stopped and the intensity of the color is measured.