Human Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) ELISA Kit

Product Overview

• Article Name: Human Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) ELISA Kit
• Biozol Catalog Number: ASC-KTE61326
• Supplier Catalog Number: KTE61326
• Alternative Catalog Number: ASC-KTE61326-48, ASC-KTE61326-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: P4HA3, C-P4H alphaI, collagen prolyl 4-hydroxylase alpha(III), procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptideI, prolyl 4-hydroxylase, alphaI subunit

Product Description

This Human Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HA3.

Product Properties

• Range: Please inquire
• Sensitivity: Please inquire
• Tag: P4HA3
• NCBI: 283208
• UniProt: Q7Z4N8
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: This Human Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HA3 in samples. An antibody specific for P4HA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP4HA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P4HA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P4HA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.