Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit

Product Overview

• Article Name: Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit
• Biozol Catalog Number: ASC-KTE61327
• Supplier Catalog Number: KTE61327
• Alternative Catalog Number: ASC-KTE61327-48, ASC-KTE61327-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: P4HA2, 4-PH alpha 2, C-P4Halpha(II), OTTHUMP00000065966, OTTHUMP00000065969, collagen prolyl 4-hydroxylase alpha(II), procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypepti

Product Description

This Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HA2.

Product Properties

• Range: Please inquire
• Sensitivity: Please inquire
• Tag: P4HA2
• NCBI: 8974
• UniProt: O15460
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: This Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HA2 in samples. An antibody specific for P4HA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP4HA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P4HA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P4HA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.