Human Matrix metalloproteinase 3 (MMP3) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP3 in samples. An antibody specific for MMP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP3 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human MMP3 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MMP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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