This Human Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) ELISA Kit employs a two-site sandwich ELISA to quantitate MKRN3 in samples. An antibody specific for MKRN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMKRN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MKRN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MKRN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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