Human Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit

Product Overview

• Article Name: Human Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit
• Biozol Catalog Number: ASC-KTE61669
• Supplier Catalog Number: KTE61669
• Alternative Catalog Number: ASC-KTE61669-48, ASC-KTE61669-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: MED13, ARC250, DRIP250, HSPC221, KIAA0593, THRAP1, TRAP240, activator-recruited cofactor 250 kDa component, mediator of RNA polymerase transcription, subunit 13 homolog, thyroid hormone receptor associate

Product Description

This Human Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit employs a two-site sandwich ELISA to quantitate MED13.

Product Properties

• Range: Please inquire
• Sensitivity: Please inquire
• Tag: MED13
• NCBI: 9969
• UniProt: Q9UHV7
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: This Human Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit employs a two-site sandwich ELISA to quantitate MED13 in samples. An antibody specific for MED13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED13 bound in the initial step. The color development is stopped and the intensity of the color is measured.