Human Mediator of RNA polymerase II transcription subunit 1 (MED1) ELISA Kit

Catalog Number: ASC-KTE61673
Article Name: Human Mediator of RNA polymerase II transcription subunit 1 (MED1) ELISA Kit
Biozol Catalog Number: ASC-KTE61673
Supplier Catalog Number: KTE61673
Alternative Catalog Number: ASC-KTE61673-48, ASC-KTE61673-96
Manufacturer: Abbkine Scientific
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: MED1, CRSP1, CRSP200, DRIP205, DRIP230, MGC71488, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2, PPAR binding protein, PPARG binding protein, thyroid hormone receptor-associated protein complex component TRAP
This Human Mediator of RNA polymerase II transcription subunit 1 (MED1) ELISA Kit employs a two-site sandwich ELISA to quantitate MED1.
Range: Please inquire
Sensitivity: Please inquire
Tag: MED1
NCBI: 5469
UniProt: Q15648
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: This Human Mediator of RNA polymerase II transcription subunit 1 (MED1) ELISA Kit employs a two-site sandwich ELISA to quantitate MED1 in samples. An antibody specific for MED1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED1 bound in the initial step. The color development is stopped and the intensity of the color is measured.