Human E3 ubiquitin-protein ligase Mdm2 (MDM2) ELISA Kit

Product Overview

• Article Name: Human E3 ubiquitin-protein ligase Mdm2 (MDM2) ELISA Kit
• Biozol Catalog Number: ASC-KTE61676
• Supplier Catalog Number: KTE61676
• Alternative Catalog Number: ASC-KTE61676-48, ASC-KTE61676-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: MDM2, HDMX, MGC5370, MGC71221, hdm2, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein, OTTHUMP00000183495, double minute 2, human homolog of, p53-binding protein, mouse double minute 2 homolog

Product Description

This Human E3 ubiquitin-protein ligase Mdm2 (MDM2) ELISA Kit employs a two-site sandwich ELISA to quantitate MDM2.

Product Properties

• Range: Please inquire
• Sensitivity: Please inquire
• Tag: MDM2
• NCBI: 4193
• UniProt: Q00987
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: This Human E3 ubiquitin-protein ligase Mdm2 (MDM2) ELISA Kit employs a two-site sandwich ELISA to quantitate MDM2 in samples. An antibody specific for MDM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.