Human Mitogen-activated protein kinase 3 (MAPK3) ELISA Kit

Product Overview

• Article Name: Human Mitogen-activated protein kinase 3 (MAPK3) ELISA Kit
• Biozol Catalog Number: ASC-KTE61732
• Supplier Catalog Number: KTE61732
• Alternative Catalog Number: ASC-KTE61732-48, ASC-KTE61732-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: MAPK3, ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3, OTTHUMP00000174538, extracellular signal-regulated kinase 1, extracellular signal-related kinase 1

Product Description

This Human Mitogen-activated protein kinase 3 (MAPK3) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK3.

Product Properties

• Range: Please inquire
• Sensitivity: Please inquire
• Tag: MAPK3
• NCBI: 5595
• UniProt: P27361
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: This Human Mitogen-activated protein kinase 3 (MAPK3) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK3 in samples. An antibody specific for MAPK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.