Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (DAD1) ELISA Kit

Catalog Number: ASC-KTE62148
Article Name: Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (DAD1) ELISA Kit
Biozol Catalog Number: ASC-KTE62148
Supplier Catalog Number: KTE62148
Alternative Catalog Number: ASC-KTE62148-48, ASC-KTE62148-96
Manufacturer: Abbkine Scientific
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: DAD1, OST2,
This Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (DAD1) ELISA Kit employs a two-site sandwich ELISA to quantitate DAD1.
Range: Please inquire
Sensitivity: Please inquire
Tag: DAD1
NCBI: 1603
UniProt: P61803
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: This Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (DAD1) ELISA Kit employs a two-site sandwich ELISA to quantitate DAD1 in samples. An antibody specific for DAD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDAD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.