Human Hepatitis B surface antigen (HBsAg) ELISA Kit employs a two-site sandwich ELISA to quantitate HBsAg in samples. An antibody specific for HBsAg has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HBsAg present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human HBsAg detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of HBsAg bound in the initial step. The color development is stopped and the intensity of the color is measured.
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