EliKine(TM) Human Prolactin ELISA Kit employs a two-site sandwich ELISA to quantitate Prolactin in samples. An antibody specific for prolactin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any prolactin present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for prolactin is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of prolactin bound in the initial step. The color development is stopped and the intensity of the color is measured.
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