EliKine(TM) Mouse CCL2 ELISA Kit

Catalog Number: ASC-KTE7001
Article Name: EliKine(TM) Mouse CCL2 ELISA Kit
Biozol Catalog Number: ASC-KTE7001
Supplier Catalog Number: KTE7001
Alternative Catalog Number: ASC-KTE7001-48, ASC-KTE7001-96
Manufacturer: Abbkine Scientific
Category: Kits/Assays
Application: ELISA
Species Reactivity: Mouse
Alternative Names: CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF, monocyte chemoattractant protein-1, monocyte chemotactic and activating factor, monocyte secretory protein JE, small inducible cytokine
EliKine(TM) Mouse CCL2 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse CCL2.
Range: 31.25 pg/mL-2000 pg/mL
Sensitivity: 16 pg/mL
Tag: CCL2
NCBI: 20296
UniProt: P10148
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: EliKine(TM) Mouse CCL2 ELISA Kit employs a two-site sandwich ELISA to quantitate CCL2 in samples. An antibody specific for CCL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL2 is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.