This Mouse Postacrosomal sheath WW domain-binding protein (WBP2NL) ELISA Kit employs a two-site sandwich ELISA to quantitate WBP2NL in samples. An antibody specific for WBP2NL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWBP2NL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WBP2NL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WBP2NL bound in the initial step. The color development is stopped and the intensity of the color is measured.
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