EliKine(TM) Mouse IL-5 ELISA Kit

Product Overview

• Article Name: EliKine(TM) Mouse IL-5 ELISA Kit
• Biozol Catalog Number: ASC-KTE7008
• Supplier Catalog Number: KTE7008
• Alternative Catalog Number: ASC-KTE7008-48, ASC-KTE7008-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Mouse
• Alternative Names: IL 5, IL5, IL-5, IL-5T-cell replacing factor, interleukin-5, TRF, TRFB cell differentiation factor I

Product Description

EliKine(TM) Mouse IL-5 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-5.

Product Properties

• Range: 7.82 pg/mL-500 pg/mL
• Sensitivity: 4 pg/mL
• Tag: IL5
• NCBI: 16191
• UniProt: P04401
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: EliKine(TM) Mouse IL-5 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-5 in samples. An antibody specific for IL-5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-5 is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound in the initial step. The color development is stopped and the intensity of the color is measured.