EliKine(TM) Mouse IL-12 p70 ELISA Kit

Product Overview

• Article Name: EliKine(TM) Mouse IL-12 p70 ELISA Kit
• Biozol Catalog Number: ASC-KTE7011
• Supplier Catalog Number: KTE7011
• Alternative Catalog Number: ASC-KTE7011-48, ASC-KTE7011-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Mouse
• Alternative Names: IL12, IL 12, IL-12, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain

Product Description

EliKine(TM) Mouse IL-12 p70 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-12 p70.

Product Properties

• Range: 31.25 pg/mL- 2000 pg/mL
• Sensitivity: 16 pg/mL
• Tag: IL12B
• NCBI: 16159
• UniProt: P43431
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: EliKine(TM) Mouse IL-12 p70 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-12 p70 in samples. An antibody specific for IL-12 p70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12 p70 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-12 p70 is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p70 bound in the initial step. The color development is stopped and the intensity of the color is measured.