EliKine(TM) Mouse TNF-alpha ELISA Kit

Product Overview

• Article Name: EliKine(TM) Mouse TNF-alpha ELISA Kit
• Biozol Catalog Number: ASC-KTE7015
• Supplier Catalog Number: KTE7015
• Alternative Catalog Number: ASC-KTE7015-48,ASC-KTE7015-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Mouse
• Alternative Names: TNF-alpha, TNF, DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein, TNF superfamily, member 2, TNF, macrophage-derived, TNF, monocyte-derived, cachectin, tumor necrosis factor alpha, TNFSF7

Product Description

EliKine(TM) Mouse TNF-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse TNF-alpha.

Product Properties

• Range: 15.63 pg/ml-1000 pg/ml
• Sensitivity: 8 pg/mL
• Tag: TNFA
• NCBI: 21926
• UniProt: P06804
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: EliKine(TM) Mouse TNF-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-alpha in samples. An antibody specific for TNF-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-alpha is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.