Mouse Nuclear factor-KB p65 (NF-KB p65) ELISA Kit

Product Overview

• Article Name: Mouse Nuclear factor-KB p65 (NF-KB p65) ELISA Kit
• Biozol Catalog Number: ASC-KTE70510
• Supplier Catalog Number: KTE70510
• Alternative Catalog Number: ASC-KTE70510-48, ASC-KTE70510-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Mouse
• Alternative Names: RELA, MGC131774, NFKB3, p65, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene

Product Description

This Mouse Nuclear factor-KB p65 (NF-KB p65) ELISA Kit employs a two-site sandwich ELISA to quantitate RELA.

Product Properties

• Range: Please inquire
• Sensitivity: Please inquire
• Tag: RELA
• NCBI: 19697
• UniProt: Q04207
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: This Mouse Nuclear factor-KB p65 (NF-KB p65) ELISA Kit employs a two-site sandwich ELISA to quantitate RELA in samples. An antibody specific for RELA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRELA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RELA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELA bound in the initial step. The color development is stopped and the intensity of the color is measured.