Mouse Acetylcholinesterase (ACHE) ELISA Kit

Catalog Number: ASC-KTE70540
Article Name: Mouse Acetylcholinesterase (ACHE) ELISA Kit
Biozol Catalog Number: ASC-KTE70540
Supplier Catalog Number: KTE70540
Alternative Catalog Number: ASC-KTE70540-48, ASC-KTE70540-96
Manufacturer: Abbkine Scientific
Category: Kits/Assays
Application: ELISA
Species Reactivity: Mouse
Alternative Names: AChE, ARACHE, N-ACHE, YT, OTTHUMP00000211347, OTTHUMP00000211349, OTTHUMP00000211356, acetylcholinesterase, apoptosis-related acetylcholinesterase
This Mouse Acetylcholinesterase (ACHE) ELISA Kit employs a two-site sandwich ELISA to quantitate AChE.
Range: Please inquire
Sensitivity: Please inquire
Tag: AChE
NCBI: 11423
UniProt: P21836
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: This Mouse Acetylcholinesterase (ACHE) ELISA Kit employs a two-site sandwich ELISA to quantitate AChE in samples. An antibody specific for AChE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAChE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AChE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AChE bound in the initial step. The color development is stopped and the intensity of the color is measured.