Anti-PDIA3 Recombinant Antibody, IgG1, Clone: [CL2444], Mouse, Monoclonal

Product Overview

• Article Name: Anti-PDIA3 Recombinant Antibody, IgG1, Clone: [CL2444], Mouse, Monoclonal
• Biozol Catalog Number: ATA-AMAB90988R
• Supplier Catalog Number: AMAb90988R
• Alternative Catalog Number: ATA-AMAB90988R-100, ATA-AMAB90988R-25
• Manufacturer: Atlas Antibodies
• Host: Mouse
• Category: Antikörper
• Application: ICC, IHC, WB
• Species Reactivity: Human
• Alternative Names: ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC

Product Description

Recombinant Mouse Monoclonal Anti-PDIA3 Antibody against Human protein disulfide isomerase family A, member 3. Validated for Immunofluorescence, Immunohistochemistry and Western Blot

Product Properties

• Clonality: Monoclonal
• Concentration: 1
• Clone Designation: [CL2444]
• Isotype: IgG1
• NCBI: 2923
• UniProt: G5EA52
• Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.

Images

Immunofluorescence staining in MCF7 cell line with Anti-PDIA3 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunohistochemistry analysis in human thyroid gland and skeletal muscle tissues using AMAb90988 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.

Immunohistochemical staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Immunofluorescence staining in A431 cell line with Anti-PDIA3 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunohistochemical staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe 1 and 2, using Anti-PDIA3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunofluorescence staining in HeLa cell line with Anti-PDIA3 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunofluorescence staining in U2OS cell line with Anti-PDIA3 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Lane 1: Marker [kDa]
Lane 2:Human cell line U-251 MG

Immunohistochemical staining of human thyroid gland shows moderate to strong cytoplasmic positivity in glandular cells.

Immunofluorescence staining in U251 cell line with Anti-PDIA3 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).