Anti-CHAT Recombinant Antibody, IgG2b, Clone: [CL3173], Mouse, Monoclonal

Product Overview

• Article Name: Anti-CHAT Recombinant Antibody, IgG2b, Clone: [CL3173], Mouse, Monoclonal
• Biozol Catalog Number: ATA-AMAB91130R
• Supplier Catalog Number: AMAb91130R
• Alternative Catalog Number: ATA-AMAB91130R-100, ATA-AMAB91130R-25
• Manufacturer: Atlas Antibodies
• Host: Mouse
• Category: Antikörper
• Application: ICC, IHC
• Species Reactivity: Human, Mouse, Rat

Product Description

Recombinant Mouse Monoclonal Anti-CHAT Antibody against Human choline O-acetyltransferase. Validated for Immunofluorescence and Immunohistochemistry

Product Properties

• Clonality: Monoclonal
• Concentration: 1
• Clone Designation: [CL3173]
• Isotype: IgG2b
• NCBI: 1103
• UniProt: P28329
• Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.

Images

Immunofluorescence staining of rat brain shows strong positivity in acetylcholine neurons in the medial septum.

Immunofluorescence staining of SK-MEL-30 cells using the Anti-CHAT monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.

Immunofluorescence staining of rat brain shows strong positivity in acetylcholine neurons in the basal forebrain.

Immunofluorescence staining of mouse brain shows strong positivity in acetylcholine neurons in the basal forebrain.

Immunohistochemistry analysis in human placenta and liver tissues using AMAb91130 antibody. Corresponding CHAT RNA-seq data are presented for the same tissues.

Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in a subset of cells in chorionic villi.

Immunohistochemical staining of human cerebral cortex shows moderate positivity in cholinergic neural fibers.

Immunofluorescence staining of mouse basal forebrain shows strong positivity in acetylcholine neurons in the caudate putamen.