Anti-POLR2F

Catalog Number: ATA-HPA000827
Article Name: Anti-POLR2F
Biozol Catalog Number: ATA-HPA000827
Supplier Catalog Number: HPA000827
Alternative Catalog Number: ATA-HPA000827-100,ATA-HPA000827-25
Manufacturer: Atlas Antibodies
Host: Rabbit
Category: Antikörper
Application: ICC, IHC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: HRBP14.4, RPB6
polymerase (RNA) II (DNA directed) polypeptide F
Anti-POLR2F
Clonality: Polyclonal
Concentration: 0.1 mg/ml
Isotype: IgG
NCBI: 5435
UniProt: P61218
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Affinity purified using the PrEST antigen as affinity ligand
Sequence: GDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGVDELII
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: POLR2F
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 0.25-2 µg/ml, IHC: 1:50 - 1:200, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & nucleoli fibrillar center.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells, cells in molecular layer and cells in granular layer.
Western blot analysis in control (vector only transfected HEK293T lysate) and POLR2F over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411849).
HPA000827-100ul
HPA000827-100ul
HPA000827-100ul