Anti-POLR3E

Catalog Number: ATA-HPA041477
Article Name: Anti-POLR3E
Biozol Catalog Number: ATA-HPA041477
Supplier Catalog Number: HPA041477
Alternative Catalog Number: ATA-HPA041477-100,ATA-HPA041477-25
Manufacturer: Atlas Antibodies
Host: Rabbit
Category: Antikörper
Application: ChIP, ICC, IHC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: FLJ10509, RPC5, SIN
polymerase (RNA) III (DNA directed) polypeptide E (80kD)
Anti-POLR3E
Clonality: Polyclonal
Concentration: 0.05 mg/ml
Isotype: IgG
NCBI: 55718
UniProt: Q9NVU0
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Affinity purified using the PrEST antigen as affinity ligand
Sequence: FVMWKFTQSRWVVRKEVATVTKLCAEDVKDFLEHMAVVRINKGWEFILPYDGEFIKKHPDVVQRQHMLWTGIQAKLEKVYNLVKETMPKKPDAQ
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: POLR3E
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 0.25-2 µg/ml, IHC: 1:200 - 1:500, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemical staining of human stomach, lower shows strong nuclear positivity in glandular cells.
Western blot analysis using Anti-POLR3E antibody HPA041477 (A) shows similar pattern to independent antibody HPA041419 (B).
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
HPA041477-100ul
HPA041477-100ul
HPA041477-100ul