Anti-POLR2I

Catalog Number: ATA-HPA045156
Article Name: Anti-POLR2I
Biozol Catalog Number: ATA-HPA045156
Supplier Catalog Number: HPA045156
Alternative Catalog Number: ATA-HPA045156-100,ATA-HPA045156-25
Manufacturer: Atlas Antibodies
Host: Rabbit
Category: Antikörper
Application: ChIP, ICC, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: hRPB14.5, RPB9
polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
Anti-POLR2I
Clonality: Polyclonal
Concentration: 0.1 mg/ml
Isotype: IgG
NCBI: 5438
UniProt: P36954
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Affinity purified using the PrEST antigen as affinity ligand
Sequence: PDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSA
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: POLR2I
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 0.25-2 µg/ml, IHC: 1:200 - 1:500, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemical staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.
Western blot analysis in human cell line HEK 293.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
HPA045156-100ul
HPA045156-100ul
HPA045156-100ul