Anti-SPC25

Catalog Number: ATA-HPA047144
Article Name: Anti-SPC25
Biozol Catalog Number: ATA-HPA047144
Supplier Catalog Number: HPA047144
Alternative Catalog Number: ATA-HPA047144-100,ATA-HPA047144-25
Manufacturer: Atlas Antibodies
Host: Rabbit
Category: Sonstiges
Application: ICC, IHC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: AD024, MGC22228, SPBC25
SPC25, NDC80 kinetochore complex component
Anti-SPC25
Clonality: Polyclonal
Concentration: 0.1 mg/ml
Isotype: IgG
NCBI: 57405
UniProt: Q9HBM1
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Affinity purified using the PrEST antigen as affinity ligand
Sequence: RLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVY
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: SPC25
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 0.25-2 µg/ml, IHC: 1:200 - 1:500, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
HPA047144-100ul
HPA047144-100ul
HPA047144-100ul