Anti-NNMT

Catalog Number: ATA-HPA059180
Article Name: Anti-NNMT
Biozol Catalog Number: ATA-HPA059180
Supplier Catalog Number: HPA059180
Alternative Catalog Number: ATA-HPA059180-100,ATA-HPA059180-25
Manufacturer: Atlas Antibodies
Host: Rabbit
Category: Antikörper
Application: ICC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: NNMT
nicotinamide N-methyltransferase
Anti-NNMT
Clonality: Polyclonal
Concentration: 0.4 mg/ml
Isotype: IgG
NCBI: 4837
UniProt: P40261
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Affinity purified using the PrEST antigen as affinity ligand
Sequence: GFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRP
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: NNMT
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 0.25-2 µg/ml, IHC: 1:50 - 1:200, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line SiHa shows localization to the Golgi apparatus.
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Western blot analysis in human cell lines U2OS and SK-MEL-30 using Anti-NNMT antibody. Corresponding NNMT RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Lane 5: Human liver tissue
HPA059180-100ul
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