Research Use Only Applications: Click Chemistry with TCO (trans-cyclooctene)-bound molecules. oYo-Link Tetrazine allows for the site-specific labeling of oYo-Link-compatible antibodies with up to two tetrazine labels per antibody for subsequent copper-free click chemistry reactions with TCO (trans-cyclooctene)-bound molecules. oYo-Link Tetrazine limits the attachment of the tetrazine labels to the heavy chains of the antibody and therefore ensures that the tetrazine label does not interfere with antibody binding. Furthermore, site-specific labeling limits 1-2 tetrazine labels to be attached per antibody, providing precise antibody-conjugate ratios. The antibody labeling procedure simply requires mixing oYo-Link Tetrazine with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table) . Requirements: To label your compatible antibodies with oYo-Link, you will need a Light Source emitting at 365nm (6-10W). Use your own Compatible Device from our list or purchase AlphaTheras LED Photo-Crosslinking Device. Technical Support: For questions concerning compatibility or click-chemistry procedure email support alphathera.com
Concentration:
270 µg/mL
Molecular Weight:
8 kDa
Buffer:
PBS
Form:
Dried pellet
oYo-Link Tetrazine Product Icon
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