Green Fluorescent Protein 4.0 mg

Catalog Number: AVL-GFP-1020
Article Name: Green Fluorescent Protein 4.0 mg
Biozol Catalog Number: AVL-GFP-1020
Supplier Catalog Number: GFP-1020
Alternative Catalog Number: AVL-GFP-1020
Manufacturer: Aves Labs
Category: Proteine/Peptide
Application: ICC, WB, IHC
Anti-Green Fluorescent Protein Antibody 4.0 mg

Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions.

Format IgY Fraction

Concentration 10 mg/mL

Clonality Polyclonal

Isotype IgY

Applications ELISA, ICC, IHC, WB

Host Species Chicken

Antigen Recombinant GFP expressed in Escherichia coli

Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Concentration: 10.0 mg/ml
UniProt: P42212
Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (RED).
Pyramidal neurons in the hippocampal formation of a neonatal mouse brain. Tissue was paraformaldehyde-fixed (4%) and paraffin-embedded. GFP staining is in green in left panel.
Comparison between GFP immunoreactivity in Rexed Lamina 2 neurons of a transgenic mouse using AVESLABS anti-GFP (GREEN) and rabbit anti-GFP (RED). The transgenic mouse was generated by placing the GFP cDNA after a POMC gene promoter in the transfected plasmid. Mark Zilka, Univ. North Carolina.
Comparison between GFP-immunoreactivity using Aves' anti-GFP antibody (LEFT panel in RED) and autofluorescence (RIGHT panel in GREEN). In this case, the cortical neuron in this unfixed thick section was first photographed for GFP autofluorescence (LEFT), and then the section was fixed (4% paraformaldehyde) and immunostained for GFP-immunoreactivity (1:1000 dilution) using Texas Red-goat anti-chicken IgY antibodies (Jackson ImmunoResearch) as a secondary. The same cell (LEFT) was then identified.