For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection range 0.6 - 200 µM in 96-well plate. Rapid and reliable. Using an optimized VCl3 reagent, the time required for reduction of nitrate (NO3-) to nitrite (NO2-) is reduced to 10 min at 60C. Simple and high-throughput. The procedure involves mixing sample with three reagents, incubation for 10 min at 60C and reading the optical density. Can be readily automated to measure thousands of samples per day. Method: OD540nm. Samples: Plasma, serum, urine, tissue/cells and foods. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.6 µM.
D2NO-100
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