For high-throughput inhibitor screening and evaluation of pyrophosphatase modulators. Key Features: Safe. Non-radioactive assay. Fast and convenient. The procedure involves incubation of enzyme and inhibitor, addition of a single working reagent and incubation for 30 min. Room temperature assay. No 37C incubator is needed. High-throughput. Homogenous ''mix-incubate-measure type assay. Can be readily automated on HTS liquid handling systems. Robust assay with a Z factor of 0.59. Can be used in 96-well, 384-well, and potentially higher density screening assays. Method: OD620, Samples: Test compounds, Species: All, Procedures: 70 min, Size: 100 tests in 96well plate
DPPI-100
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