This special red-fluorescent dye enables advanced click reaction kinetics due to the usage of a picolyl-azide. This structure can complex copper and, therefore, the dye is already connected to its catalyst for the efficient click reaction. One benefit is that reducing copper concentration allows click reaction with sensitive reactants. Picolyl azides enhance the reaction kinetics so much that it is possible to have a picolyl structure that reacts selectively with an alkyne even when other azides are present.[1] This enables among other things the usage of less amount of copper in the click reaction mixture without any effect on the great reaction efficiency achieving the same reaction yields. This effect can be crucial e.g. when copper sensitive enzyme or biomolecules shall be labeled. Once attached to a biomolecule via click chemistry (CuAAC, copper catalyzed alkyne-azide cycloaddition) fluorescence microscopy detection can be performed similar to standard dyes. Due to the spectral properties the Eterneon-Red 645 picolyl dye can be used as AF647 alternative and Atto 647 alternative.
Molecular Weight:
953.15 g/mol
Purity:
95% (HPLC)
Form:
Solid
Formula:
C43H52N8O11S3
Application Notes:
Fluorescent labeling via click chemistry
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