5-Ethynyl uridine (EU) is a modified nucleoside that can be used for labeling of nascent RNA inside cells. After feeding EU, it can react with azides. Our own experiments indicate that it will be first integrated in de novo synthesized RNA, but we have also observed incorporation into DNA after some incubation time. This is most likely via conversion of the EU ribonucleoside into EdU aided by intracellular ribonucleotide reductases. Research from several groups demonstrates successful EU cellular labeling for divers applications.
Molecular Weight:
268.22 g/mol
Purity:
98% (HPLC)
Form:
Solid
CAS Number:
[69075-42-9]
Formula:
C11H12N2O6
Application Notes:
Stabilizing Triphosphate which enhance the RNA Transfection
5-Ethynyl uridine (5-EU)
5-Ethynyl uridine (5-EU)
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