Modified Triphosphate for labeling of 3END RNA or ssDNA
C8-Alkyne-dUTP is easily incorporated into DNA by PCR instead of dUTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by click chemistry. We recommend a substitution of 1 - 10% of dUTP.
Concentration:
100 mM
Molecular Weight:
572.29 g/mol
Purity:
95% (HPLC)
Form:
Liquid
CAS Number:
[1004297-65-7 free acid]
Formula:
C17H23N2O14P3
Application Notes:
C8-Alkyne-dUTP is a modified deoxyuridine triphosphate (dUTP) featuring a C8 alkyne group at the C5 position of the uracil base. It enables site-specific DNA labeling via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC), supporting a wide range of downstream applications in molecular biology, diagnostics, and nanobiotechnology.
C8-Alkyne-dUTP
C8-Alkyne-dUTP
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