Modified Triphosphate for labeling of 3END RNA or ssDNA
5-Ethynyl-dUTP (5-EdUTP) is easily incorporated into DNA by PCR instead of dUTP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by Click Chemistry. We recommend a substitution of 1 - 10% of dUTP.
Concentration:
100 mM
Molecular Weight:
492.16 g/mol
Purity:
95% (HPLC)
Form:
Liquid
CAS Number:
[111289-87-3]
Formula:
C11H15N2O14P3
Application Notes:
Enzymatic incorporation into DNA by PCR to produce ethynyl-functionalized DNA
5-Ethynyl-dUTP (5-EdUTP)
5-Ethynyl-dUTP (5-EdUTP)
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