Alkyne-dATP (7-deaza-7-Ethynyl-dATP) is easily incorporated into DNA by PCR instead of dATP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by click chemistry. We recommend a substitution of 1 - 10% of dATP.
Concentration:
10 mM
Molecular Weight:
514.22 g/mol
Purity:
98% (HPLC)
Form:
Liquid
CAS Number:
[1208963-36-3 free acid]
Formula:
C13H17N4O12P3
Application Notes:
Efficient synthesis of 5'-cap mimics by combining two nucleotide units, resulting in a linear dinucleotide cap analog, with a free clickable moiety.
Alkyne-dATP (7-deaza-7-Ethynyl-dATP)
Alkyne-dATP (7-deaza-7-Ethynyl-dATP)
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